Welcome to the PSI SGKB Technology Portal:

The PSI Technology Portal presents methods and technologies catalyzed by the NIH Protein Structure Initiative (PSI) high-throughput efforts. Many of these technologies are of benefit to many levels of research, and instructions are given here on how the technologies can be obtained and used.

To begin your search, either enter terms into the search box, click on an experimental step within the Pipeline diagram below, or select a specific center.

If you have any questions about this site, please contact the administrator at psi-tech@lbl.gov.

Featured technology: reconstitution of a three-component membrane protein complex using wheat germ cell-free technology

Wheat germ cell-free translation has been shown to be an effective method to produce integral membrane proteins in the presence of unilamelar liposomes. A three-component membrane protein complex was reconstituted using wheat germ cell-free technology. Our data strongly supports the utility of wheat germ cell-free technology to produce active and correctly folded integral membrane proteins [details].